DNA实验--免疫细胞表面抗原分子CD家族对照表.docx

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1、免疫细胞外表抗原分子CD家族对照表(CD1CD247)ExalphaBiologicalsproducesandsellsproductsforLifeScienceResearch,includingmonoclonalandpolyclonalantibodies,foruseinimmunohistochemistry,westernblot,ELISAandFlowCytometry.Weofferover800productsand,throughcollaborationswithotherscientistsaswellasourownin-houseR&Dprograms,Ex

2、alphacontinuestobringleadingedgeproductstomarket.OurfamilyofCDantibodiescombinesqualityandperformancewithreasonablepricestomakeourCDantibodiesthebestvalueinthemarkettoday.OurfamilyofProliferationproductsoffereasyall-in-onekitsorstandaloneantibodiestoquantitateproliferatingcells.Takealookatourwebsite

3、orcallusforadditionalinformation.NoteinoinformationisavailableonCDw145,CD181,CD182,CD185-194,CD196-199.CD211,CD214-216,CD218-220,CD237BackgroundCDxlusterdesignationofmonoclonalantibodies(clustersofdifferentiation)Designatedatthe1stto7thWorkshopsonInternationalHumanLeukocyteDifferentiationAntigensThe

4、lastconferencewasin2000.Thenextconferenceisin2004.HLDAWorkshopsaretheprimarymechanismtocharacterizeleukocytesurfaceantigenicmoleculesandepies;erythroidantigensarenowincludedFor1stto6thworkshops,antibodiesweresubmittedtotheorganizinglaboratory,codedandsenttoparticipatinglaboratoriesfortestingagainstv

5、ariouscelltypes.Forthe7thworkshop,aCDdesignationcouldbeestablishedforamoleculeifitsgenehasbeenclonedandatleastonespecificmonoclonalantibodyhadbeenstudiedintheWorkshopInterpretationshouldbebasedoncellulardistributionofstaining,proportionofpositivelystainedcells,stainingintensityandcutofflevels.CD1Fam

6、ilyofnon-polymorphicMHCclassI-IikeglycoproteinsAlsomemberofimmunoglobulinsuperfamilyOnchromosomelq22-23(notMHClinked)Has5differentsubsets,allnoncovalentlyassociatedwith12kdbeta2microglobulinFunctionirestrictTcellresponsestocertainantigens;maymediatethymicTcelldevelopmentPositivestaining(normal):cort

7、icalthymocytes(70%),activatedTcells,Langerhanscells,interdigitatingdendriticcellsPositivestaining(disease):preTALLwithcorticalthymocytephenotype;LangerhanscellhistiocytosisNegativeStainingimatureperipheralTcellsCDlaPositivestaining(normal):Dendriticcellsindermis/epidermisofbenigninflammatoryskindiso

8、rdersPositivestaining(disease)angerhanscellhistiocytosis(fairlyspecific),myeloidleukemias,someBcellmalignancies;dendriticcellsinmostperipheralcutaneousTcellIymphomassAJCP2001;116:72NegativeStainingznormalBcells,mostcutaneousperipheralBcelllymphomas(?reflectsreplacementofreactivepatterncontainingdend

9、riticcellswithaneoplasticpatternofBcells)MicroImageszLangerhanscellhistiocytosisMicroimages(AJSPsubscribers):pulmonaryLangerhanscellhistiocytosisMicroimages(HumPathsubscribers)angerhanscellhistiocytosisReferenceszAJSP2001;25：630CDlbPositivestaining(disease):myeloidleukemiasandsomeBcellmalignanciesNe

10、gativeStainingznormalBcellsCDlcPositivestaining(normal):subsetofnormalperipheralBcellsPositivestaining(disease):myeloidleukemiasandsomeBcellmalignanciesNegativeStainingmormalBcellsCDldPositivestaining(normal):thymus(lowlevels),bowelCDleCD2AkaErosettereceptor,LFA-2(leukocytefunctionantigen)Functionzb

11、indsCD58/LFA-3onantigen-presentingcells,andinducescostimulatorysignalsinTcellsAlsoregulatesTandNK-mediatedcytolysis,inhibitsapoptosisofactivatedperipheralTcells,mediatesTcellcytokineproduction,regulatesTcellanergyPositivestaining(normal):thymocytes(95%),matureperipheralTcells(almostall),NKcells(80-9

12、0%),thymicBcells(50%)Microimages:extranodalNK/Tcelllymphoma,nasaltypeCD2RCD2epiesrestrictedtoactivatedTcellsPositivestainingactivatedTcells,?NKcellsCD3AkaOKT3Functionzcomplex(5chains)ofintegralmembraneglycoproteinsassembledasacomplex;haslongcylasmictailwithantigenrecognitionactivationmotif;complexis

13、thendownregulatedAlsosubdividedintodelta,epsilon,gammasubtypesCylasmicexpressionatearlyTcelldifferentiation,thenmembranousexpressionMostspecificTcellantibodyPositivestaining(normal):thymocytes,peripheralTcells,NKcells;alsoPurkinjecellsofcerebellumPositivestaining(disease):80%ofTcelllymphomasNegative

14、staining:gammadeltaTcellreceptors,mostBcelllymphomasMicroimages:CD3epsilon-testicularNK/Tcelllymphoma(figure3D)Microimages(AJSPsubscribers):achalasia,post-transplantlymphoproliferativediseaseinliverReferenceszAJSP2001;25：1413CD4Aka0KT4,Thelper/inducerOnchromosome#12pNonpolymorphousglycoproteinsbelon

15、gingtoimmunoglobulinsuperfamilyServesasHIVreceptoronTcells(asdoChemokinereceptorsCCR5andCXCR4),macrophages,brainCD4+TcellsarekilledbyHIVCoreceptorinMHCclass!1-restrictedantigeninducedTcellactivationBindstononpolymorphicregionofclassImolecules;mayincreaseavidityofcell-cellinteractionsPositivestaining

16、(normal):thymocytes(80-90%),matureTcells(65%,ThelperandCD4CD8+thymocytes),macrophages,Langerhanscells,dendriticcells,granulocytesPositivestaining(disease):pityriasislichenoidesMicroimages:acutedemyelinatingdisease,extranodalNK/Tcelllymphoma,nasaltypeCD5BelongstoancientscavengerreceptorfamilyIsphysic

17、allyandfunctionallycoupledwithTcellreceptor-zeta-CD3signaltransducercomplexCD5+Bcellsproduce“generalistantibodies*-polyreactivelowaffinitynatural*antibodiestoexogenousantigens(tetanustoxoid,lipopolysaccharide)aswellasautoreactiveantibodies(ssDNA,thyroglobulin,insulin)Note:sharksonlyhavepolyreactiveI

18、gMNote:monoreactiveIgGisproducedby0.1%ofcirculatingBcells,frompositiveselectionandsomaticpointmutationFirstlineofdefenseagainstantigens;havealowactivationthreshold;aretheonlylineofdefenseforthosewhocannotproducespecificantibodyProduceantibodiesusinggermline(nonmutated)configurationofgenesegments,usu

19、allyIgMProductionelevatedinrheumatoidarthritis(27-52%ofcirculatingBcellsvs.20%normal)CD5mayserveasadualreceptor,givingeitherstimulatoryorinhibitorysignalsdependingbothonthecelltypeandthedevelopmentstagePositivestaining(normal):Bcellsofmantlezoneofspleenandlymphnodes;Bcellsinperitonealandpleuralcavit

20、ies;almostallTcells;Infetus,mostBcellsinspleenandcordbloodareCD5positivePositivestaining(disease):BcellCLL/SLL,mantlecelllymphoma,mostTmalignancies,thymiccarcinomas(70%)NegativeStainingrspindlecellthymomas,MALTlymphoma,follicularlymphomaMicroImageszextranodalNK/Tcelllymphoma,nasaltype,mantlecelllymp

21、homa(figure3D)CD6AdhesionmoleculemediatingthebindingofdevelopingthymocyteswiththymicepithelialcellsMaybeinvolvedinautoimmunityandgraftvs.hostdisease(GVHD)AntibodiestoCD6areusedtodepleteTcellsfrombonemarrowtransplantstopreventGVHDPositivestaining(normal)dowlevelsonimmaturethymocytes,highlevelsonmatur

22、ethymocytesCD7MembraneglycoproteinandFcreceptorforIgMHomologoustoTCRgamma,IgkappaMembraneexpressionearlyduringTontogeny,beforeTCRrearrangement,persistsuntilterminalstagesofTcelldevelopment1.owerexpressioninmemoryTcellsvs.naiveTcellsPositivestaining(normal):matureperipheralTcells(85%)lpost-thymicTcel

23、ls(majority),NKcells(majority),somemyeloidcellsPositivestaining(disease):!cellALL;AML(especiallyM4M5),chronicmyelogenousleukemia,blastsintransientmyeloproliferativedisorderNegativeexpression:BcellALL,Sezarysyndrome,adultTcellIeukemiaZIymphomaMicroimages:extranodalNK/Tcelllymphoma,nasaltypeCD8Aka0KT8

24、,Tcellsuppressor/cytotoxiccellsOnchromosome#2MHCclassIrestrictedreceptor;bindstononpolymorphicregionofclassImolecules;mayincreaseavidityofcell-cellinteractionsAssociatedwithIymphoepithelioma-Iikecarcinomaoflung(AJSP2002;26:715)Positivestaining(normal):Tcells(25-35%ofmatureperipheralTcells,mostcytoto

25、xicTcells,CD4CD8+thymocytes);NKcells(30%-whicharealsoCD3negative);corticalthymocytes(70-80%),epidermotrophiclymphocytesinmycosisfungoides(AJSP2002;26:450)Microimagesdymphoepithelioma-likecarcinomaofcervix-figure3,acutedemyelinatingdiseaseMicroimages(ModPathsubscribers):nodalcytotoxicTcelllymphomaRef

26、erencezModPath2002;15:1131CD9MaymediateplateletactivationandaggregationAntibodiesareusedtopurgebonemarrowpriortoperipheralstemcellbonemarrowtransplantViralco-receptorPositivestaining(normal):preBcells,Bcellsubset,Tcells,macrophages,platelets,eosinophils,basophils,megakaryocytes,endothelialcells,brai

27、n,peripheralnerve,vascularsmoothmuscle,cardiacmuscle,epitheliaCDlOAkacommonAcuteLymphoblasticLeukemiaAntigen(CALLA),neutralendopeptidase24.11,neprilysin,enkephalinaseCellmembranemetallopeptidase,characteristicmarkeroffollicularcentercellsandfollicularlymphoma,butalsowidelydistributedinnormaltissuean

28、dneoplasms;alsolocalizedtobrushborderinsmallbowelmucosaInactivatesbioactivepeptidesUses:AcutelymphoblasticIeukemiazoneoffirstmarkerstoidentifyleukemiccellsinchildren(henceitsname)Breastimarkerofmyoepithelialcells,ModPath2002;15:397BurkittIymphomaxonfirmdiagnosisColonicCarcinogenesisdncreaseinstromalcellsfrommildtoseveredysplasiatoinvasiveCarcinoma1HumPath2002;33:806-811Endometriosiszhelpfulinidentifyingareasofendometriosisifsparseglandulartissue